Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1, TNF-, IL-6, and HMGB1 Expression
Figure 6
Modulatory effects of HOCbl on NOS activity in tissue homogenates of endotoxemic mice at 4 h and 24 h. Liver ((a), (c)) and lung (b) tissue samples were collected 4 h and 24 h after LPS challenge (0.1 mg/kg; i.p.); two groups of mice ( per group) having previously been treated with HOCbl (resp., 0.2 mg/kg i.p. at −1 h, +1 h, and +2 h and at −1 h, +1 h, +2 h, +6 h, and +22 h) and also challenged with LPS at time 0. NOS activity at 4 h and 24 h was assayed as described in Materials and Methods, and shown as µmol of NO generated per µg protein. Values are a mean SEM of triplicate observations. versus vehicle (PBS-treated) control; versus LPS group.
