Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1, TNF-, IL-6, and HMGB1 Expression
Figure 7
The effects of thiol Cbls on activity of NOS. Liver tissue samples were collected 4 h after LPS challenge (0.1 mg/kg; i.p.); 4 groups of mice ( per group) having previously been treated with either LPS-only or HOCbl/or GSCbl or NAC-Cbl plus LPS at time 0 h (0.2 mg/kg i.p. at −1 h, +1 h, and +2 h and at −1 h, +1 h, +2 h, +6 h, and +22 h). NOS activity at 4 h was assayed as described in Materials and Methods, and shown as µmol of NO generated per µg protein. Values are a mean SEM of triplicate observations. versus vehicle (PBS-treated) control; versus LPS group.
