Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1, TNF-, IL-6, and HMGB1 Expression
Figure 8
Selective effects of HOCbl on IL-6 and TNF-alpha at 4 h following LPS-induced endotoxaemia. Mice () were treated with HOCbl, at the low dose protocol (0.2 mg/kg i.p.) at −1 h, +1 h, and +2 h and compared to LPS (0.1 mg/kg i.p. at time 0). Organs (lung and liver) were harvested at 4 h subsequent to LPS, and gene expression quantified in tissue extracts by real-time PCR, using GAPDH and RPL32 as internal standards. ELISA was used to measure plasma levels of IL-6 (a) and TNF- (b). Tissue expression of TNF- mRNA in lung (c) and liver (d) extracts. Data are a mean SEM of 5 mice per group. versus vehicle (PBS-treated) control; versus LPS group.
