Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1𝜷, TNF-𝜶, IL-6, and HMGB1 Expression

Table 1

Protocol for cobalamin treatment of LPS-induced endotoxaemia.

Experimental protocols	Inflammatory stimulus	Treatment
		Cbl dose	Time of Cbl treatment
4 h non-lethal	LPS (0.1 mg/kg)	0.2 mg/kg	−1 h, +1 h, +2 h
24 h non-lethal	LPS (0.1 mg/kg)	0.2 mg/kg	−1, +1, +2, +6, +22 h
Lethal series I	LPS (0.1 mg/kg)	0.2 mg/kg	−1, +1, +2, +6, +22 h
	+ D-Gal (1 g/kg)	40 mg/kg	+2, +22 h
Lethal series II	LPS (0.1 mg/kg)	40 mg/kg HOCbl	+2, +4 h
	+ D-Gal (1 g/kg)	80 mg/kg HOCbl	+2 h