Follistatin-Like 1: A Potential Mediator of Inflammation in Obesity
Figure 1
FSTL1 expression is downregulated during differentiation of 3T3-L1 preadipocytes. (a) FSTL1 mRNA expression was evaluated by quantitative RT-PCR in the course of 3T3-L1 preadipocyte differentiation and in RAW264.7 cells. Relative mRNA expression was normalized to β-actin and expressed as fold of D0 value. (b) FSTL1 protein expression in differentiating preadipocytes was assessed by Western blot analysis (top) and quantified by densitometry (bottom). Relative protein expression was normalized to GAPDH and expressed as fold of D0 value. Data are mean ± SE; . * versus undifferentiated preadipocytes (D0).
