Cigarette Smoke Suppresses the Surface Expression of c-kit and FcεRI on Mast Cells

Figure 2

CSM modulates surface expression of FcεRI and c-Kit. BMMCs were cocultured in presence or absence of CSM (1.5%) or LPS during the third week of bone marrow culture. The surface expression of c-kit (upper panels) and FcεRI (lower panels) was detected by flow cytometry (blue histograms): control (a), CSM (b), and LPS (c). Green histograms represent isotype controls. (d) Quantification of 3 representative FACS analyses showing the mean fluorescence intensity (MFI) for each group. Values are expressed as mean ± S.E.M (𝑛=3). ^∗,#𝑃<0.05 is significantly different (increased/decreased) compared to control.

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