Cigarette Smoke Suppresses the Surface Expression of c-kit and FcεRI on Mast Cells
Figure 4
Long-term exposure of mast cells to CSM inhibits cytokine production by mast cells. BMMCs (control, CSM, and LPS cultured for 4–6 days) were sensitized with DNP-specific IgE, followed by activation with dinitrophenyl-conjugated ovalbumin (DNP-Ova) for 9 h. The levels of cytokines IL-4 (a), IL-5 (b), IL-6 (c), IL-13 (d), and TNF-α (e) in the supernatants were estimated by ELISA. Data are mean ± SEM of quadruplicate samples. The asterisks represent significant differences between nonactivated and activated cells (). Hatches represent significant differences between control mast cells and mast cells cocultured with CSM or LPS ( and ).
