CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production
Figure 1
Interference with LPS/CD14 interaction moderately affects TNF-α production induced by high concentrations of sLPS or rLPS. RAW264 cells were pretreated with 10 μg/mL of the anti-CD14 antibody, clone 4C1, or isotype-matched control rat IgG2b (30 min, 37°C) and subsequently stimulated with 1–1000 ng/mL sLPS (a, b) or 1–1000 ng/mL rLPS (c, d) in the presence or absence of 10% FBS, as indicated. Concentrations of TNF-α (a, c) and RANTES (b, d) were measured by ELISA tests in supernatants of the cells. Data shown are mean ± SEM form three or four experiments each run in triplicate. *Significantly different from cells stimulated with a corresponding LPS concentration in the presence of control IgG and FBS.
