CD14 Mediates Binding of High Doses of LPS but Is Dispensable for TNF-α Production
Figure 3
Effect of neutralizing CD14 on cytokine production during activation of TLR2/TLR6, TLR2/TLR1, and TLR3. RAW264 cells were pretreated with 10 μg/mL of the anti-CD14 antibody or isotype-matched control rat IgG2b (30 min, 37°C) and exposed to indicated concentrations of Pam2CSK4 (a) or Pam3CSK4 (b) or poly(I:C) (c) in the presence of 10% FBS. Amounts of TNF-α (a, b) and RANTES (c) were measured by ELISA tests in supernatants of the cells. Data shown are mean ± SEM form two or three experiments each run in triplicate. *Significantly different from cells stimulated with a corresponding ligand concentration in the presence of control IgG.
