The Effect of Anti-Inflammatory and Antimicrobial Herbal Remedy PADMA 28 on Immunological Angiogenesis and Granulocytes Activity in Mice
Figure 1
Effect of PADMA 28 (0.085 or 5.8 mg) supplementation of Balb/c donors on the number of newly formed blood vessels measured by lymphocyte-induced angiogenesis test (LIA) in F1 (Balb/c × C3H) recipient mice. Balb/c mice were fed daily per os with 0.085 mg or 5.8 mg of PADMA 28. After 7 days, mice were bled and sacrificed, and splenocytes were isolated. Splenocytes were pooled within the groups, resuspended in Parker culture medium (1 million of cells in 0.05 mL), and injected intradermally (5-6 injections per mouse) to 24 F1 (Balb/c × C3H) hybrids. After 72 hours the mice were euthanized, and newly formed blood vessels were counted in dissection microscope (6x magnification). Results are shown as mean SEM. ***.
