Contribution of CFTR to Alveolar Fluid Clearance by Lipoxin A4 via PI3K/Akt Pathway in LPS-Induced Acute Lung Injury
Figure 3
Effects of lipoxin A4 on protein expression of CFTR in vivo. Lipoxin A4 (2 μg/kg) was administered intravenously to Sprague-Dawley (SD) rats 6 h after LPS (20 mL/kg) stimulation through the tail vein, and intratracheal instillation of 5% albumin solution containing Evans Blue-labeled albumin through a tracheostomy to the left lung, ventilating for 1 hour. The right lung was isolated. (a) The protein expression of CFTR was assessed by immunohistochemistry. Immunopositive cells were counted in five randomly selected nonoverlapping fields of three separately immunostained lung sections per group. (b) The right lung was also homogenized for western blotting. Data were expressed as mean ± SEM for each group. * versus control group, # versus LPS group. LPS = lipopolysaccharide, LXA4 = lipoxin A4, CFTR = cystic fibrosis transmembrane conductance regulator.
