E. coli Endotoxin Modulates the Expression of Sirtuin Proteins in PBMC in Humans

Figure 2

Protein expression of SIRT1, SIRT2, SIRT3, IκB-β, phospho-IκB-β, PBEF, and β-actin in isolated PBMC incubated with LPS. An upregulation of SIRT1 protein expression was detected 24 hours following LPS. SIRT2 protein expression was unchanged. SIRT3 decreased 3 hours after LPS. IκB-β expression was decreased 3 hours after LPS, and phosphorylated IκB-β was upregulated following LPS administration. PBEF showed first a decrease after 3 and 6 hours and an increase after 24 hours following LPS.