JNK and NADPH Oxidase Involved in Fluoride-Induced Oxidative Stress in BV-2 Microglia Cells
The activation of microglial BV-2 cells induced by fluoride. Cells were treated with indicated concentrations of NaF for 24 h and immunofluorescence localization with an Iba-1 antibody as a microglial marker being observed. Microglial activity was detected by Iba-1 expression (red) in cytoplasm and nucleus dyed with DAPI was presented in blue. Highly expression areas of Iba-1 immunoreactivity were indicated by red colour. Fluorescence microscopy: (400x). (a) Control, (b) 1 mg/L NaF, (c) 5 mg/L NaF, (d) 10 mg/L NaF, (e) 50 mg/L NaF, and (f) 100 mg/L NaF.