Nitric Oxide Is a Mediator of Antiproliferative Effects Induced by Proinflammatory Cytokines on Pancreatic Beta Cells
Figure 4
Role of different NOS isoforms in cytokine-induced antiproliferation in cultured β cells. (a) and (c) iNOS and eNOS expression was assessed using western blot analysis in pancreatic islets cultured over 48 h under basal conditions and treated with a mixture of cytokines IL-1β (50 U/mL) + IFN-γ (1000 U/mL) + TNF-α (1000 U/mL) (CTKS) over 6, 12, 24, and 48 h. A representative image is shown (Cont: positive brain control in eNOS Western blot). (b) Representative image of iNOS (green) and insulin (red) immunostaining of control and cytokines treated (CTKS) pancreatic islets cultured over 48 h. (d), (e), and (f) rat islets cultured over 48 h in the presence of 7 Ni (e), 1400 W (d), and L-NIO (f) alone or with the mixture of cytokines (CTKS). Results are presented as percentage means ± SEM of combined BrdU/insulin-positive cells relative to insulin-positive cells in a minimum of 5 experiments. treated versus control.
