Resolution of PMA-Induced Skin Inflammation Involves Interaction of IFN- and ALOX15
Figure 2
Neutralization of endogenous IFN- led to accelerated reduction of epidermal thickness and epithelial cell proliferation. (a) Mice were treated with isotype control mAb (control) or anti-IFN- mAb (anti-IFN-) after the third PMA treatment. Then, mice from control and anti-IFN- groups were sacrificed 3 hours, 4 days, or 7 days after the third PMA treatment. (b) HE-stained skin sections of different groups were shown. Statistical analysis of the epidermal thickness of skin from normal (without PMA treatment), control, or anti-IFN- groups was measured as described in Methods (5 fields per section, 5 or 6 mice per group) and presented in (c) Scale Bar, 50 μm. , compared to control group. (d) Skin sections were stained with anti-Brdu (red) to display the proliferating cells. The boundary between epidermis and dermis is marked by a dotted line. Scale Bar, 200 μm. (e) Skin sections of different groups were stained with Sirius-Red binding to all types of collagen and Fast-Green binding to noncollagenous proteins (magnification as (b)).
