Celecoxib Improves Host Defense through Prostaglandin Inhibition during Histoplasma capsulatum Infection
Figure 2
Celecoxib treatment increases the phagocytosis of yeast by alveolar macrophages in vivo and NO production in the lung parenchyma. (a) The BALF cells from mice infected with a sublethal inoculum of H. capsulatum, with or without celecoxib treatment, were collected and stained after cytospin preparation. The yeast cells in the alveolar macrophages were counted using bright-field microscopy at 100x magnification. The phagocytic index was calculated as described in Section 2. (b) The production in the lung parenchyma was measured using Griess reagent. The data are presented as the means ± SEM representative of three independent experiments . One-way ANOVA (Tukey's multiple comparison test) was used. *PBS + H2O versus Hc + H2O or versus Hc + celecoxib; #Hc + H2O versus Hc + celecoxib .
