VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue
Figure 1
CM-induced proliferation is mediated by VEGF. (a) VSMC were serum starved for 24 h and subsequently incubated with CM, 100 μmol/L OA, or the combination of CM and OA in the presence of BrdU for 18 h. Incorporation of BrdU into DNA was measured. Data are expressed relative to control, taken as 100%. Data are mean values ± SEM of three independent experiments. * compared to control or designated treatment. (b) The proliferative effect of CM correlates significantly with its VEGF content as measured by ELISA. Statistical result of linear regression analysis is indicated in the graph. (c) VSMC were serum starved for 24 h and subsequently incubated with 250 pg/mL VEGF, 100 μmol/L OA, or the combination of VEGF and OA in the presence of BrdU for 18 h. Incorporation of BrdU into DNA was measured. Data are expressed relative to control, taken as 100%. Data are mean values ± SEM of three independent experiments. * compared to control or designated treatment. (d–f) VSMC were treated with CM, OA, and their combination for 24 h. Total cell lysates were resolved by SDS-PAGE and immunoblotted with specific VEGF-R1 and -2 antibodies. A representative blot is shown. Data are mean values ± SEM of three independent experiments. All data were normalized to the level of actin expression and are expressed relative to the control.
