Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study

Table 2

Characteristics of primers used for real-time QRT-PCR.

Gene	Sequence of primers	Length of amplicon (bp)*	Tm (°C)^†
IGF1	Forward: 5′-TGCTTCCGGAGCTGTGATC-3′	221	77.6
	Reverse: 5′-GATCCTGCGGTGGCATGTCACTCTTCACT-3′
IGF1R	Forward: 5′-ACGCCAATAAGTTCGTCCACAGAGACCT-3′	187	77.4
	Reverse: 5′-GAAGACTCCATCCTTGAGGGACTCAG-3′
IGFBP3	Forward: 5′-GCTACAGCATGCAGAGCAAGT-3′	102	82.4
	Reverse: 5′-CAGCTGCTGGTCATGTCCTT-3′
GAPDH	Forward: 5′-GAAGGTGAAGGTCGGAGTC-3′	226	80.0
	Reverse: 5′-GAAGATGGTGATGGGATTC-3′

∗bp: base pairs.
†Tm: melting temperature.