5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils
Effect of eosinophil transfer into GATA-1 recipients on neutrophil accumulation. (a)–(d) Eosinophil-deficient GATA-1 mice and wild-type (WT) controls (BALB/c) were injected with RPMI (open bars) or eotaxin, 50 ng/cavity (black bars). The peritoneal lavage fluid collected after 4 h was used for quantitation of total leukocytes (a), eosinophils (b), macrophages (c), and neutrophils (d). E-H, GATA-1 mice received eotaxin (black bars), BALB/c eosinophils (stippled bars), or BALB/c eosinophils followed by eotaxin administration 30 minutes later (hatched bars). Peritoneal lavage fluid collected after 4 h of eotaxin administration was used for quantitation of total leukocytes (e), eosinophils (f), macrophages (g), and neutrophils (h). Data are mean ± SEM. *, 0,05; **, 0,01, for the indicated differences. Data from 3–11 experiments. (i) Intensity of CCR3 expression in granulocytes. Cells were collected 4 h after eotaxin injection from the peritoneal cavity of GATA-1 and BALB/c donors and stained for CCR3. Representative MFI profiles for the granulocyte gate are shown. Dotted line, GATA-1. Thin line, BALB/c. Thick line, GATA-1 sample to which purified BALB/c eosinophils were added in vitro (up to 20% of total cells).