Mediators of Inflammation / 2014 / Article / Fig 8

Research Article

5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils

Figure 8

Flow cytometric analyses of peritoneal lavage leukocytes. (a)–(c) Interaction between fluorescent bacteria and neutrophils from eotaxin-injected and control mice. BALB/c mice were injected with eotaxin 50 ng/cavity i.p. Controls received RPMI. After 4 h, peritoneal lavage fluid was collected from both groups. After counting neutrophils, fluorescent, viable E. coli bacteria were mixed with leukocytes at a 400 : 1 bacteria/neutrophil ratio and further incubated for 30 min before washing to eliminate unbound bacteria, and analysis of neutrophil-associated fluorescence by flow cytometry. (a) Representative profiles of eotaxin-induced (thick, continuous line) and RPMI-induced (thin, interrupted line) neutrophil-associated fluorescence. (b) Fraction of the neutrophils positive for fluorescent bacteria in RPMI-induced (open bar) and eotaxin-induced (black bar) peritoneal leukocyte populations ( ); (c) mean fluorescent intensity of neutrophils in the same samples (mean ± SEM). Data from 4-5 experiments.