Figure 5: Effect of DEC on immunohistochemical localization TNF-α in lung after carrageenan-induced pleurisy. (a) In tissue sections obtained from the CAR group, positive staining for TNF-α was mainly located in inflammatory cells. (b) After treatment with DEC, the degree of positive staining for TNF-α was reduced in the lung tissue. (c) Densitometry analysis of immunohistochemistry photographs for TNF-α in lung tissues; figure representative of at least 3 experiments performed on different experimental days; data expressed as mean ± S.E.M. from mice for each group; ND: not detected; versus carrageenan; scale bar = 20 μm.