Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA

Table 2

Patients with positive BC, ST, or IHP. Bacterial infection was considered confirmed when plausible pathogens were found in blood culture and by molecular methods.

Case	Comorbidities	Conventional microbiology	ST	IHP	Bacterial infection
1	Acute cholecystitis	Klebsiella pneumoniae (blood culture and respiratory tract)	Klebsiella pneumoniae	Klebsiella pneumonia	Confirmed
2	Alcoholic liver cirrhosis	Staphylococcus aureus (blood culture)	Staphylococcus aureus	Negative	Confirmed
3	Knee prosthesis	Staphylococcus aureus (blood culture and knee joint aspirate)	Staphylococcus aureus	Negative	Confirmed
4	Congestive heart failure, generalized atherosclerosis	Negative	Streptococcus pneumoniae	Streptococcus pneumoniae	Plausible pathogen
5	Congestive heart failure, diabetes type II	Negative	Streptococcus pneumoniae	Streptococcus pneumoniae	Plausible pathogen
6	Diabetes type II, chronic obstructive pulmonary disease	Negative	Streptococcus pneumoniae	Negative	Plausible pathogen
7	Injection drug use, endocarditis	Negative	Streptococcus salivarius	Negative	Plausible pathogen
8	Chronic renal failure, diabetes mellitus	Negative	Staphylococcus hominis	Negative	Considered contamination
9	Colon cancer	Negative	Streptococcus mitis group	Negative	Considered contamination