Ecto-nucleotidases Activities in the Contents of Ovarian Endometriomas: Potential Biomarkers of Endometriosis
Figure 4
Enzyme activity in exosome-enriched fractions from simple cysts and from endometriomas. (a) ATPase (black circles) and ADPase (white circles) activities of increasing amounts of exosomes from endometriomas (0, 5, 10, 20, 30, and 50 μg). (b) Ectonucleotidase activity, represented as pmoles of generated Pi, in exosome-enriched fractions from simple cysts (white columns) and from endometriomas (gray columns) in the presence of ATP, ADP, or AMP as substrate. All the activities are significantly higher in endometriomas than in simple cysts (). (c) Western blot with anti-CD39 and anti-CD73 antibodies of exosome-enriched fractions from an endometrioma. Molecular weight markers in kDa are indicated at the left of each image.
