Anti-Inflammatory Mechanism of Polyunsaturated Fatty Acids in Helicobacter pylori-Infected Gastric Epithelial Cells
Figure 6
Effects of PUFAs on PKCδ activation in H. pylori-stimulated AGS cells. (a) AGS cells were infected with H. pylori for the indicated time points (4, 8, 15, 30, and 60 min). Phosphospecific and total forms of PKCδ were determined by western blotting. (b) AGS cells were pretreated with various concentrations of rottlerin (0.04, 0.2, and 1 M), a specific PKCδ inhibitor, for 1 h and then cultured with H. pylori for 30 min. Phosphospecific and total forms of ERK and JNK in whole cell extracts were determined by western blotting. (c) AGS cells were pretreated with individual fatty acid (100 M) for 24 h and were then cultured with H. pylori for 8 min.
