Myeloperoxidase Oxidized LDL Interferes with Endothelial Cell Motility through miR-22 and Heme Oxygenase 1 Induction: Possible Involvement in Reendothelialization of Vascular Injuries

Figure 2

The effect of Mox-LDL treatment on HUVEC tubulogenesis. (a) Representative fields of HUVEC cultures in tubulogenic conditions. HUVECs were seeded on Matrigel supplemented with mock medium (CTL), native LDL (LDL), or Mox-LDL (MoxLDL) and incubated overnight. The fields are representative of three independent experiments performed in duplicate. (b) Quantification of tubulogenesis in HUVEC cultures. After overnight incubation in Matrigel supplemented with mock medium (CTL), native LDL (LDL), or Mox-LDL (Mox-LDL), pictures were taken of 4.906 mm square fields. Total tubule network length was then measured using the Image J computer program. Shown data are expressed as total tubule network length for each condition and normalized to the untreated control set as 100%. Mean ± SEM on 3 independent experiments performed in duplicate. versus control (ANOVA, Turkey’s multiple comparison test).

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