Myeloperoxidase Oxidized LDL Interferes with Endothelial Cell Motility through miR-22 and Heme Oxygenase 1 Induction: Possible Involvement in Reendothelialization of Vascular Injuries

Figure 5

Effect of Mox-LDL treatment on HUVEC proliferation. (a) A representative Flow cytometry analysis of HUVEC cells incubated for 24 hours in culture medium supplemented with mock medium, native LDL, or Mox-LDL. Cells were then harvested and analyzed for the dead cell stain and the Ki67 proliferation marker. Percentages of cells located in the different quadrants are indicated. (b) Statistical analysis of the percentages of proliferating cells determined as in (a) on 3 independent experiments performed in duplicate. Mean ± SEM (ANOVA, Turkey’s multiple comparison test). Effect of Mox-LDL treatment on HUVEC cell death. (c) A representative flow cytometry analysis of HUVEC cells incubated for 24 hours in culture medium supplemented with mock medium, native LDL, or Mox-LDL. Cells were then harvested and analyzed for PI DNA staining and phosphatidylserine annexin V staining. Percentages of cell located in the different quadrants are indicated. The results are representative of three independent experiments performed in duplicate. (d) Statistical analysis of the percentages of PI-negative annexinV positive cells determined as in (c) on 3 independent experiments performed in duplicate. Mean ± SEM (ANOVA, Turkey’s multiple comparison test).

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