Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1
Figure 5
P2X7 receptors expression in peritoneal macrophages. (a) Macrophages from uninfected (white bar) and S. mansoni-infected (black bar) mice express similar levels of P2X7 protein. replicates from three different animals, for each group. The images above are representative Western blots (U = uninfected and I = infected). (b) P2X7 receptors expression in peritoneal macrophages from control mice (white bar, untreated) or TGF-β1-treated cells (5 ng/mL, 24 h; black bar) are also similar. The images above are representative Western blots (U = uninfected; TGF-β1= TGF-β1-treated macrophages). Lysates from P2X7 KO macrophages were used as negative controls for P2X7 protein expression. Cells lysates were obtained from three animals for each group. (c) Quantitative RT-PCR (qRT-PCR) analysis of P2X7 receptor mRNA levels in peritoneal macrophages. Similar levels of P2X7 mRNA were found in uninfected (white bar), S. mansoni-infected mice (black bar), and TGF-β1-treated (5 ng/mL, 24 h; hatched bar) mouse macrophages. samples per group (using different animals).
