Effect of Tumor Necrosis Factor Family Member LIGHT (TNFSF14) on the Activation of Basophils and Eosinophils Interacting with Bronchial Epithelial Cells
Figure 2
Effect of LIGHT on the cell surface expression of ICAM-1 on BEAS-2B cells or basophils/eosinophils. Expressions of ICAM-1 on BEAS-2B cells alone or in the coculture, and basophils/eosinophils in the coculture with or without LIGHT stimulation are presented with representative bar charts. (a) Representative histogram of the cell surface expression of ICAM-1 on BEAS-2B cells (1 × 105 cells) treated with different concentration of LIGHT (0–100 ng/mL) for 24 h is shown. (b) Basophils or (c) eosinophils (3 × 105 cells) and confluent BEAS-2B cells (1 × 105 cells) were cultured either together or separately with or without LIGHT (1–100 ng/mL) for 24 h. Surface expressions of ICAM-1 on 5,000 BEAS-2B cells, basophils, or eosinophils are expressed as the mean plus SEM of MFI of three independent experiments with three blood samples. *, ***. (d) Basophils or (e) eosinophils (0.3 × 105–3 × 105 cells) and confluent BEAS-2B cells (1 × 105 cells) were cultured together with or without LIGHT (100 ng/mL) and transwell (pore size 0.4 μm) for 24 h. Surface expressions of ICAM-1 on 5,000 BEAS-2B cells, basophils, or eosinophils are expressed as the mean plus SEM of MFI of three independent experiments with three blood samples. *; ** when compared with the cocultured BEAS-2B cells and eosinophils without stimulation with LIGHT (empty bar). # when compared with the corresponding group without transwell inserts. BS: BEAS-2B cells alone; CoBS: BEAS-2B cells in the coculture; CoBAS: basophils in the coculture; CoEosi: eosinophils in the coculture.
