Ecotin-Like ISP of L. major Promastigotes Fine-Tunes Macrophage Phagocytosis by Limiting the Pericellular Release of Bradykinin from Surface-Bound Kininogens: A Survival Strategy Based on the Silencing of Proinflammatory G-Protein Coupled Kinin B2 and B1 Receptors
Figure 2
Differential role for bradykinin receptors in the phagocytic response of macrophages infected by WT L. major promastigotes and mutants. Thioglycolate elicited (peritoneal) macrophages were incubated in medium containing 1 mg/mL BSA in the presence or absence of 100 nM of HOE-140 or 1 μm of des-Arg9-[Leu8]-BK (DAL8-BK). Promastigotes were added (parasite/cell ratio 5 : 1) and incubated for 3 h at 37°C. White bars represent L. major WT and black bars represent . Data represent numbers of intracellular amastigotes per 100 macrophages (means ± SD) for triplicates and represent two different experiments ().
