Early Activation of Pulmonary TGF-β1/Smad2 Signaling in Mice with Acute Pancreatitis-Associated Acute Lung Injury

Figure 1

Expression of three different isotypes of TGF-β in the lungs. Immunostaining of TGF-β1 (a and b), TGF-β2 (c and d), and TGF-β3 (e and f) levels in lung tissue from sham controls (a, c, and e) and 9 h after acute pancreatitis induction (b, d, and f). Positive staining is visualized in brown by DAB and counterstained in blue by haematoxylin. Results from one representative of eight samples per group are shown. Original magnification, ×20. Western blot analysis of TGF-β1 protein levels in lung extracts of sham-operated and ligated animals at time points indicated (g). Two of five similar analyses per group are shown. Graph shows densitometry evaluation of fold difference in TGF-β1 intensity compared to sham control and represents mean ± SEM of five samples per group (h). *𝑃<0.05; **𝑃<0.01.

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