Early Activation of Pulmonary TGF-β1/Smad2 Signaling in Mice with Acute Pancreatitis-Associated Acute Lung Injury
Figure 3
Distribution of Smad proteins in the lungs. Immunostaining of Smad2 (a and b), Smad3 (c and d), and Smad4 (e and f) levels in lung tissue at 9 h from sham controls (a, c, and e) and acute pancreatitis groups (b, d, and f). Results from one representative of eight samples per group are shown. Original magnification, ×20. Western blot analysis of Smad2, Smad3, and Smad4 protein levels in lung extracts of sham-operated and ligated animals at the indicated time points (g). Two of five similar analyses per group are shown. Graphs show densitometry measurements of fold difference in Smad2, Smad3, and Smad4 levels compared to sham control and represent mean ± SEM of five samples per group (h).
