Early Activation of Pulmonary TGF-β1/Smad2 Signaling in Mice with Acute Pancreatitis-Associated Acute Lung Injury

Figure 5

Redistribution and nuclear translocation of P-Smad2. Representative images of P-Smad2 in lung tissue at 9 h (a and b) and 24 h (c and d) following sham operation (a and c) and acute pancreatic induction (b and d). Original magnification, ×20. Arrows indicate nuclear translocation of P-Smad2 after 24 h in the ligated animals (f). More cytoplasmic (arrows) and less nuclear staining of P-Smad2 were observed in the sham-operated group (e). Original magnification, ×40. Results from one representative of eight samples per group are shown. Western blot analysis of total P-Smad2 protein levels in lung extracts of sham-operated and ligated animals (g). Two of five similar analyses per group are shown.