Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors

Figure 2

Real-time PCR results evaluating mRNA levels of inflammatory pathway related genes in kidney transplant patients. IL-1R1, IFNA1, HMOX-1, TGF-𝛽, IL-10, and IL-1F7 were assayed by real-time PCR in whole kidney RNA samples from no DGF patients and DGF patients. Values were normalized to the level of 𝛽-actin RNA. Relative expression levels of all genes were calculated as 2^{^}[(Ct reference gene) − (Ct target gene)]. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 by Mann Whitney test.

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