Resolution of Sterile Inflammation: Role for Vitamin C

Figure 6

Macrophages deficient in Vitamin C have reduced antioxidant capacity. Peritoneal macrophages elicited on day 3 following TG-induced peritonitis from VitC sufficient ((a)–(d)) and deficient Gulo^−/− ((e)–(h)) mice were exposed to 12.5, 25, and 50 μM for 18 hours and probed with MitoTracker Red CMXRos. Macrophages from some VitC deficient mice were incubated with AscA (3 mM, 16 hours) prior to exposure to H₂O₂ followed by staining with MitoTracker Red CMXRos (Deficient + AscA, ((i)–(l))).

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