Mediators of Inflammation

Research Article

Generation of IL-8 and IL-9 Producing CD4+ T Cells Is Affected by Th17 Polarizing Conditions and AHR Ligands

Table 2

Absolute concentration of cytokines (pg/mL) secreted by CD4+ T cells cultured under activation (anti-CD3/anti-CD28), Th17 polarizing conditions (TGF-β, IL-1β, IL-23), and with the AHR ligands FICZ, TCDD, and B[a]P measured by CBA (IL-17A, IL-9, IL-8, and IFN-γ) and ELISA (IL-22).
Cytokine Time after activation (days)Cytokine concentration (pg/mL), mean ± SEM,  
CD4+ T cell culture conditions
Anti-CD3/anti-CD28+Th17 polarizing condition+FICZ (100 nM)+TCDD (5 nM)+B[a]P (100 nM)
IL-17A1
2
3
4
5
IL-221n.a.n.a.n.a.n.a.n.a.
2n.a.n.a.n.a.n.a.n.a.
3
4n.a.n.a.n.a.n.a.n.a.
5
IL-91
2
3
4
5
IL-81
2
3
4
5
IFN-γ1
2
3
4
5
represents significance compared to anti-CD3/anti-CD28, .
Mann-Whitney test, and .
#represents significance compared to Th17 polarizing conditions, , Mann-Whitney test, and .
n.a.: not analyzed.