The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls

Table 2

Scheme of in vitro stimulation of mononuclear cells.

	Cells; (10^7 cells/mL)	Stimulation	Cultivation X-Vivo; medium
PWM	100 L	100 L PWM	800 L
PWM + ConA	100 L	100 L PWM + 100 µL ConA	700 L
HSP70	100 L	100 L	800 L
A.a.	100 L	100 L A.a.	800 L
P.g.	100 L	100 L P.g.	800 L
T.f.	100 L	100 L T.f.	800 L
Unstim.	100 L		900 L

PWM: Pokeweed mitogen, ConA: Concanavalin A, HSP70: Heat Shock Protein 70, A.a.: Aggregatibacter actinomycetemcomitans, P.g.: Porphyromonas gingivalis, T.f.: Tannerella forsythia, and unstim.: without stimulation.