Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis
Figure 2
(a) NF-κB reporter activation is suppressed by gambogic acid treatment. NF-B expression was measured by flow cytometry in a THP-1/NF-B reporter cell line incubated with gambogic acid and then stimulated for 24 h with E. coli. Each thin line in the histogram corresponds to untreated but E. coli stimulated cells, the shaded area corresponds to drug-treated and E. coli stimulated cells, and the thick line corresponds to untreated non-stimulated cells as a control. (b) Caspase-1 activation is decreased with gambogic acid treatment. Caspase-1 activation was measured using flow cytometry in a THP-1 cell line incubated with gambogic acid and then stimulated for 8 h with E. coli. Each thin line in the histogram corresponds to untreated but E. coli stimulated cells used as control and the thick line corresponds to drug-treated and E. coli stimulated cells.
