Protective Effect of Baccharis trimera Extract on Acute Hepatic Injury in a Model of Inflammation Induced by Acetaminophen

Figure 1

RP-UPLC-DAD profiles of hydroethanolic extract of B. trimera. Conditions: CHS130 100 RP-18 column (1.7 μm, 50 × 3 mm i.d.). Elution was carried out with a linear gradient of water 0.1% formic acid (A) and acetonitrile 0.1% formic acid (B) (from 5% to 95% of B in 11 min) and the UPLC fingerprints were registered on a ACQUITY Waters apparatus with a UV-DAD detector (Waters 2996). Operating parameters of the mass spectrometer were capillary temperature 320°C; spray needle voltage set at 3.50 kV; ES capillary voltage +3 and −47 V for positive and negative polarity, respectively; and tube lens offset 0 and −25 V for positive and negative polarity, respectively. Nitrogen was used as a sheath gas with a flow of 50 arbitrary units. Mass analysis was carried out in full-scan mode from 100 to 1.500 amu, in both positive and negative mode. UV spectra (190–450 nm) from the main peaks are shown inside on the chromatogram.