Secretoglobin Superfamily Protein SCGB3A2 Deficiency Potentiates Ovalbumin-Induced Allergic Pulmonary Inflammation
Figure 4
Cytokine expression levels in the lungs and BALF. (a) IL-4, IL-5, and IL-13 expression levels were determined by qRT-PCR for lung mRNA levels (upper panel) and ELISA for protein levels in BALF (lower panel) from control (Saline) and OVA challenged (OVA) mice and (+/+) mice. (b) Lung mRNAs, encoding IL-9, CCR3, CCR4, CCL11, CCL17, and CCL22 from control (Saline) and OVA-challenged (OVA) mice and (+/+) mice, were quantified by qRT-PCR. (c) IL-4, IL-5, and IL-13 expression levels were determined by qRT-PCR for lung mRNA levels (upper panel) and ELISA for protein levels in BALF (lower panel) from control (Saline) and OVA challenged (OVA) mice and (+/+) mice. (d) Lung mRNAs encoding IL-9, CCR3, CCR4, CCL11, CCL17, and CCL22 from control (Saline) and OVA-challenged (OVA) and (+/+) mice were quantified by qRT-PCR. qRT-PCR results were normalized with Ppia and the relative expression levels were expressed based on the value of saline-challenged wild-type lungs as 1. , , , and by one-way ANOVA.
