Figure 3: The effect of EBN on IL-6, MCP-1, and COX-2 levels in DSS-induced colitis mice. COX-2 levels were measured in colon tissue lysates by using western blot analysis (a). β-Actin was used as an internal control. Serum levels of IL-6 and MCP-1 were measured using enzyme-linked immunosorbent assays (ELISAs) ((b) and (c)). The levels of IL-6, MCP-1, and COX-2 are shown for the untreated, DSS-treated, DSS + EBN 100, DSS + EBN 200, and DSS + EBN 500 groups. All data are expressed as the mean ± SD. versus DSS-treated; # versus untreated.