Mediators of Inflammation / 2014 / Article / Fig 1

Research Article

Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases

Figure 1

Frequencies and absolute counts of circulating Treg and Th17 cells as well as the ratio of Th17/Treg in CTD and CTD-aPAH patients and healthy controls. PBMCs from studied subjects were stained with labeled anti-human antibodies as described in Section 2. Flow cytometry dot-plots showed that the gating strategies were used in identification of Treg (gated on CD4+ cells for detection of CD4+ CD25+ Foxp3+ and CD4+ CD25+ CD127 cells) and Th17 cells (gated on CD4+ cells for detection of CD4+ IL-17+ cells). (a) The dot-plots showed the gating strategies of circulating CD4+ CD25+ Foxp3+ Treg, CD4+ CD25+ CD127 Treg and Th17 cells in control, CTD and CTD-aPAH groups. (b) Statistical analysis revealed the frequencies of CD4+ CD25+ Foxp3+ Treg and CD4+ CD25+ CD127 Treg populations among the CD4+ T cells in healthy controls () and in patients with CTD () or CTD-aPAH (). (c) Frequencies (left) and absolute counts (right) of circulating Treg cells (CD4+ CD25+ Foxp3+) in control, CTD and CTD-aPAH groups. (d) Frequencies (left) and absolute counts (right) of circulating Th17 cells in control, CTD and CTD-aPAH groups. (e) The ratios of Th17/Treg in control, CTD and CTD-aPAH groups. (f) Th17 and Treg cells frequencies and the ratios of Th17/Treg in subgroups of mild to moderate CTD-aPAH and severe CTD-aPAH. Compared with control group, ; Compared with CTD group, .
247372.fig.001a
(a)
247372.fig.001b
(b)
247372.fig.001c
(c)
247372.fig.001d
(d)
247372.fig.001e
(e)
247372.fig.001f
(f)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.