In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure
Figure 4
Analysis of circulating cytokines/chemokines in sera of HF patients in relationship to the in vitro endothelial cell proliferation response. Cytokines/chemokines levels were measured in sera of HF patients by multiplex immunoassay. In (a), the levels of the indicated cytokines measured in HF patients clustered in “high” and “low” (based on the in vitro endothelial cell index) are expressed as means ± SD. * and . In (b), correlation between the normalized cell proliferation index are recorded at 36 and 48 hours and the levels of IL-8 (upper panels) and VEGF (lower panels) are evaluated in the HF patients’ serum samples. Correlation coefficients (), calculated by Spearman’s analysis, and values are reported for each correlation.
