Mediators of Inflammation

Research Article

In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure

Table 1

Circulating levels of cytokines/chemokines in sera from healthy subjects.


Cytokines/chemokines	Limit of detection	Healthy subjects		P
Cytokines/chemokines	Limit of detection	Young	Old	P

EGF	3.21	236 ()	142 ()	<0.05
EOTAXIN	3.2	79.5 ()	136 ()	<0.05
G-CSF	3.24	22 ()	28.9 ()	n.s.
GM-CSF	1.58	3.2 ()	4 ()	n.s.
IFN-2	0.72	3.8 (	5.1 ()	<0.05
IFN-	1.37	1.8 ()	4.2 ()	n.s.
IL-10	0.89	<OOR	<OOR	/
IL-12p40	0.36	<OOR	<OOR	/
IL-12p70	1.6	<OOR	<OOR	/
IL-13	0.82	<OOR	<OOR	/
IL-15	1.06	<OOR	<OOR	/
IL-17	1.28	<OOR	<OOR
IL1ra	0.36	4.8 ()	4.2 ()	n.s.
IL-1	0.87	<OOR	<OOR	/
IL-1	0.81	<OOR	<OOR	/
IL-2	1.44	<OOR	<OOR	/
IL-3	/	<OOR	<OOR	/
IL-4	1.17	<OOR	<OOR	/
IL-5	1.04	<OOR	<OOR	/
IL-6	0.63	20.2 ()	<OOR	n.s.
IL-7	0.62	1.8 ()	3.3 ()	<0.001
IL-8	1.51	3.7 ()	8.1 ()	<0.001
IP-10	3.21	197 ()	173 ()	n.s.
MCP-1	3.21	436 ()	573 ()	n.s.
MIP-1	0.9	2.7 ()	3.7 ()	<0.001
MIP-1	3.21	30.2 ()	34.1 ()	n.s.
TNF-	1.26	5.03 ()	5.4 ()	n.s.
TNF-	1.31	<OOR	<OOR	/
VEGF	3.2	59.8 ()	155 ()	<0.001

Values are expressed as pg/mL and reported as median (mean ± SD). OOR<: out of (below) detection range; n.s: nonstatistically significant; : statistically significant.