Relationship of MMP-14 and TIMP-3 Expression with Macrophage Activation and Human Atherosclerotic Plaque Vulnerability
Figure 1
(a) mRNA expression of M-CSF differentiated macrophages (MM) and foam-cell macrophages (FCM) after activation with IFNγ and LPS (MM1 and FCM1) or IL-4 (MM2 and FCM2). Data are ; ; compared to MM and MM2; compared to FCM and FCM2; compared to MM and MM1; and compared to FCM and FCM1. ((b)–(d)) Densitometric quantification and representative Western blots for MMP-14, COX-2, TIMP-3, and CD206 in M-CSF differentiated macrophages (MM) or after activation with IFNγ and LPS (MM1) or IL-4 (MM2). Data are , compared to MM, . ((e)-(f)) Densitometric quantification and representative Western blots for MMP-14 (e) and TIMP-3 (f) in M-CSF differentiated macrophages (MM) and foam-cell macrophages (FCM). Data are , compared to MM, compared to MM, . (g) QPCR expression of miR-24 in M-CSF differentiated macrophages (MM) and foam-cell macrophages (FCM). Data are , compared to MM. ((h)-(i)) Densitometric quantification and representative Western blots for MMP-14 (h) and TIMP-3 (i) in foam-cell macrophages (FCM) after activation with IFNγ and LPS (FCM1) or IL-4 (FCM2). Data are ; .
