Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor-κB Phosphorylation In Vitro and In Vivo
Figure 1
Effects of lipopolysaccharide (LPS) exposure on proinflammatory cytokine expression in human bronchial epithelial cells. Cells from the (a, b) HBEC and (c, d) BEAS-2B lines were incubated with various concentrations of LPS for various durations, and proinflammatory cytokine expression was evaluated using western blotting. Tumor necrosis factor-α (TNF-α), interleukin- (IL-) 1β, and IL-8 proteins expression were upregulated in a dose-dependent manner. Data are presented as means ± standard error of the mean (SEM) from 3 independent experiments. Each bar graph shows summarized data from 3 separate densitometry experiments after normalization to α-tubulin (an internal control for cytoplasm protein). versus LPS dose 0 or time 0.
