Induction of Heme Oxygenase-1 with Hemin Reduces Obesity-Induced Adipose Tissue Inflammation via Adipose Macrophage Phenotype Switching

Figure 3

HO-1 induction alters macrophage polarization in cocultures. Raw264.7 macrophages were seed onto 3T3-L1 adipocytes with/without being pretreated with the indicated concentrations of hemin or CORM-2 for 12 h. (a) IL-4 levels in hemin/CORM-2-treated cocultures. (b) Total RNAs were isolated and the level of IL-4 was analyzed by qRT-PCR. Transcript levels of the M2 markers Mrc1 (c) and Clec10a (d) and the M1 marker (CD274) (e) were detected by qRT-PCR; β-actin was used as control gene. The experiment was performed in duplicate. Results are means ± SEM. , , , and . A.U.: arbitrary units.

(a)

(b)

(c)

(d)

(e)