Nucleotides Regulate Secretion of the Inflammatory Chemokine CCL2 from Human Macrophages and Monocytes
Figure 8
P2Y2 expression is similar in macrophages expressing 312Arg-P2Y2 and 312Ser-P2Y2 variants. (a) An in-house genotyping assay was designed using PCR and restriction enzyme analysis with MwoI. Individuals with GG (wild-type, lanes 4, 5, and 6) have a single upper band of 195 bp. Individuals with CC (homozygous for 312Ser, lanes 7 and 8) show a single upper band of 181 bp. Individuals with GC genotype (heterozygous for 312Ser, lanes 1, 2, and 3) show two upper bands at 195 and 181 bp. (b) Flow cytometry dot plots showing gating of CD14 positive PBMCs and representative histograms showing P2Y2 expression on CD14+ gated population.
