The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice
Figure 4
Effect of Rg1 on the RANKL-induced expression of c-Fos, c-Jun, and NFATc1 in RAW 264.7 cells. (a) RAW 264.7 cells (1 × 105 cells/mL) were plated in a 6 cm culture plate and cultured with or without RANKL (50 ng/mL) and/or Rg1 (100 μg/mL) for indicated periods. The cell lysates were subjected to Western blot analysis with an antibody to c-Fos, c-Jun, and NFATc1. (b) RAW 264.7 cells (1 × 105 cells/mL) were plated in a 6 cm culture plate, and RAW264.7 cells were cultured with RANKL (50 ng/mL) and/or increasing concentrations of Rg1 for 48 h. The cells were subjected to Western blot analysis. (c) RAW264.7 cells (2 × 104 cells/mL) were cultured with or without RANKL (50 ng/mL) and/or Rg1 (1, 10, and 100 μg/mL) for 48 h in 12-well plates. The total cellular RNA was extracted and used for RT-PCR analysis to detect c-Fos, c-Jun, and NFATc1 mRNA. All data are representative of three different experiments.
