Mediators of Inflammation / 2014 / Article / Fig 6

Research Article

Oral Administration of Herbal Mixture Extract Inhibits 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in BALB/c Mice

Figure 6

Effect of CP001 on PMA plus A23187-stimulated proinflammatory cytokine expression in HMC-1. HMC-1 were pretreated with various concentrations of CP001 for 1 h and then treated with PMA and A23187 for 24 h. The levels of IL-6 and IL-8 in culture supernatants were measured by ELISA assay (a), (c). The IL-6 and IL-8 mRNA levels were measured by RT-PCR (b), (d). Data represent the mean ± SEMs of three independent experiments.