Osteopontin Expression in the Brain Triggers Localized Inflammation and Cell Death When Immune Cells Are Activated by Pertussis Toxin

Figure 2

Proinflammatory molecules modified by OPN. QRT-PCR was used to evaluate levels of chemokine receptors and proinflammatory molecules using SABiosciences PCR array, and SyBr Green/ROX detectors in an ABI HT7900 Fast apparatus. We measured 84 genes involved in inflammatory responses, including chemokines and receptors (PAMM-022Z, Qiagen). Results show the genes that were significantly changed. (a) Genes upregulated in OPN-injected brain lobes compared to β-gal controls. (b) Genes downregulated in OPN compared to β-gal controls. Results represent average ± SD of 6 experimental lobes injected with either β-gal or OPN-encoding vector. Experiments were performed in triplicate. (-test) in comparison to β-gal. Immunohistochemistry was utilized for validation. (c) Immunohistochemistry for tissue detection of CXCR3 in representative sections of the vector injection site, in β-gal and Ptx group, and in (d) OPN-encoding vector and Ptx.

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